Laser-induced multidimensional fluorescence spectroscopy in Shpol'skii matrixes for the analysis of polycyclic aromatic hydrocarbons in HPLC fractions and complex environmental extracts.

Fluorescence measurements are easily made with the aid of a cryogenic fiber optic probe. Emission wavelength time matrixes, excitation emission matrixes, and time-resolved excitation emission matrixes are rapidly collected with a pulsed tunable dye laser, a spectrograph, and an intensified charged-coupled device. Compound identification is based on spectral and lifetime information. The potential of this approach for supporting high-performance liquid chromatography analysis and for the direct determination of benzo[a]pyrene without previous separation is demonstrated.